Abstract
Objective To investigate the effects of relationship between ventrolateral preoptic nucleus (VLPO) and tuberomammillary nucleus (TMN) on the regulation of sleep-wake cycle in rats. Methods SD male rats were randomly divided into control group (TMN+ ACSF&VLPO+ ACSF) and experimental groups (TMN+ ACSF&VLPO+ L-Glu, TMN+ Bic & VLPO+ ACSF, TMN+ Bic&VLPO+ L-Glu). Brain stereotaxic technique, microinjection and polysomnography were used to detect and record the variation of rat sleep. Results Compared with the TMN+ ACSF&VLPO+ ACSF group (n=8), the amount of wakefulness declined (42.30±3.75, P<0.01) along with the enhancement of non-rapid eye movement (NREM) sleep (61.97±3.41, P<0.01) and total sleep time (TST) (77.68±3.74, P<0.01) after L-Glu microinjected into VLPO (n=7), while there was no significant change in rapid eye movement (REM) sleep. Compared to those of L-Glu microinjected into VLPO alone in the TMN+ ACSF&VLPO+ L-Glu group (n=7), the wakefulness increased (79.22±3.93, P<0.01), meanwhile, NREM sleep (34.82±3.23, P<0.01), REM sleep (5.97±1.10, P<0.05) and TST (40.79±3.92, P<0.01) decrease after L-Glu microinjected into VLPO followed by the Bic microinjected into TMN (n=8). Conclusion VLPO can inhibit the neuronal activity of TMN via GABAA receptor and induce the sleep-promoting effect. Key words: Ventrolateral preoptic nucleus; Tuberomammillary nucleus; Bicuculline; Sleep; Wakefulness
Published Version
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