Abstract
ObjectiveTo discuss the clinical value of immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma.MethodsA total of 209 cases of B-cell lymphomas and 35 cases of reactive lymphoid hyperplasia were selected for DNA extraction and PCR amplification using the BIOMED-2 primer system. Gel electrophoresis of heteroduplexes was used to analyze immunoglobulin gene rearrangements.ResultsA total of 209 cases of B-cell lymphoma, including 69 extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue, 63 diffuse large B-cell lymphomas, 39 follicular lymphomas, 15 small lymphocytic lymphomas, 6 plasmacytomas, 6 mantle cell lymphomas, 7 nodal marginal zone B-cell lymphomas, and 4 lymphoplasmacytoid lymphomas, were examined. Immunoglobulin gene rearrangements were found in all 209 cases, with 93 IGHA, 122 IGHB, 98 IGHC, 167 IGK, 100 IGL, 167 IGHA/B/C, 204 IGH/IGK, 209 IGH/IGK/IGL, 129 IGH+IGK, 81 IGH+IGL, 83 IGK+IGL and 68 IGH+IGK+IGL gene rearrangements. Immunoglobulin gene rearrangements were not found in the 35 cases of reactive lymphoid hyperplasia. IGH and IGK gene rearrangements were mainly found in mantle cell lymphomas, small lymphocytic lymphomas, extranodal marginal zone B-cell lymphomas of mucosa-associated lymphoid tissue and diffuse large B-cell lymphomas. The IGH gene rearrangement was mainly found in lymphoplasmacytoid lymphomas and follicular lymphomas. IGK and IGL gene rearrangements were mainly found in plasmocytoma, and the IGK gene rearrangement was mainly found in nodal marginal zone B-cell lymphomas.ConclusionsThe BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis. Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL.
Highlights
Lymphoma is a malignant tumor derived from lymphoid tissue and lymph nodes, which can be classified into two types, Hodgkin lymphoma (HL) and nonHodgkin lymphoma (NHL) [1, 2]
The BIOMED-2 standardized immunoglobulin gene rearrangement detection system is an important tool in B-cell lymphoma diagnosis
Analysis of IGH, IGK and IGL gene rearrangements is valuable in confirming the classification of B-cell NHL
Summary
Lymphoma is a malignant tumor derived from lymphoid tissue and lymph nodes, which can be classified into two types, Hodgkin lymphoma (HL) and nonHodgkin lymphoma (NHL) [1, 2]. In the late 1980s, gene analysis was utilized as an auxiliary diagnostic method together with histopathology and immunophenotyping analysis, as it could provide an alternative way for the diagnosis of malignant clones of lymphocytes. With the development of molecular biology, gene analysis has become an important supplementary method in the diagnosis of malignant lymphoma. Detection of gene rearrangements in immunoglobulin (IG), a specific marker of B lymphocyte clones, is an important method in the diagnosis of B-cell lymphoma [3,4,5,6,7]. To explore the clinical value of IG gene rearrangements in the diagnosis of B-cell lymphoma, the BIOMED-2 primer system was used for the analysis of IG gene rearrangements
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