Abstract
The validation of the method of DNA extraction from Apis mellifera L. tissue was the aim of our work. The honeybee samples were harvested from the apiary of the Department of Technology of Honeybee Rearing from the University of Agricultural Sciences and Veterinary Medicine Cluj–Napoca in August 2007. DNA was extracted according to the protocol proposed by Hunt et al., 1999 and modified by Wilkes and Oldroyd, 2003. The NANODROP device was used for purity measurements. In order to elaborate the validation protocol the following parameters were calculated: detection limit, quantification limit, repeatability, reproducibility and uncertainty.
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