Abstract
Forensic identification through DNA analysis is an accurate diagnostic tool. Deoxyribonucleic Acid (DNA) analysis is via DNA repetitive regions with less than 1 kb base size is called ‘microsatellite’ or Short Tandem Repeat (STR). At the crime scene, the perpetrator’s skin may accidentally be in contact with surrounding objects, thereby transferring trace evidence to the objects. In this study DNA was obtained using “touch DNA” from two buccal smears and two smear from watches and cellphones from volunteers who had signed the consent form. Samples were isolated using DNAzol. The quantity of DNA obtained will be measured using a UV spectrophotometer. For DNA amplification using 3 STR CODIS loci namely TH01, CSF1PO, and TPOX. The last step is visualization using acrylamide gel and silver staining. Mean levels of DNA (UVVisible Spectrophotometer) were 167.89±85.71 μg/mL for the buccal swab, 59.19±5.58 μg/mL for the watch swab, and 38.09±2.12 μg/mL for the mobile swab; the purity of the buccal swab DNA was 1.79±0.71, of the watch swab 1.69±0.76, and of the mobile swab 1.53±0.56. Visualization of PCR products on Polyacrylamide Agarose Composite Gel Electrophoresis stained with Silver and amplified using the standard primers THOI, TPOX and CSF1PO for STR Combined DNA Index System (CODIS) showed a 100% detection of amplicons. Both the buccal swab, watch swab and handphone swabs had trace amount of DNA that was sufficient to be isolated and amplified by using Polymerase Chain Reaction on the STR CODIS loci THO1, CSF1PO and TPOX.
Highlights
Deoxyribonucleic Acid (DNA) identification is a way of identifying individuals through characteristics and features that distinguish them from others
At the crime scene the perpetrator’s skin surface or part of his body is often accidentally exposed to the surrounding objects, resulting in the transfer of trace evidence to such objects. In this case one of the technologies, namely touch DNA/contact trace DNA, can be used, through the DNA that is transferred in the form of skin cells when objects are held or touched.[5]
Research conducted by Yudianto et al concluded that there are external factors that connect the environment and the duration of exposure to the quantity and quality of DNA from to thank all the volunteers and the Human Genetics and Forensic Laboratory of the Institute for Tropical Diseases, Universitas Airlangga, Surabaya, Indonesia
Summary
Deoxyribonucleic Acid (DNA) identification is a way of identifying individuals through characteristics and features that distinguish them from others. S Deoxyribonucleic Acid (DNA) analysis is u via DNA repetitive regions with less than 1 kb base size is called ‘microsatellite’ or Short l Tandem Repeat (STR). At the crime scene the perpetrator’s skin surface or part of his body is often accidentally exposed to the surrounding objects, resulting in the transfer of trace evidence to such objects In this case one of the technologies, namely touch DNA/contact trace DNA, can be used, through the DNA that is transferred in the form of skin cells when objects are held or touched.[5] Research conducted by Yudianto et al concluded that there are external factors that connect the environment and the duration of exposure to the quantity and quality of DNA from to thank all the volunteers and the Human Genetics and Forensic Laboratory of the Institute for Tropical Diseases, Universitas Airlangga, Surabaya, Indonesia. ©Copyright: the Author(s), 2020 Licensee PAGEPress, Italy Infectious Disease Reports 2020; 12(s1):8716 doi:10.4081/idr.2020.8716
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