Abstract
Recent studies have shown the ability of electrochemical methods to sense and determine, even at very low concentrations, the presence and quantity of molecules or analytes including pharmaceutical samples. Furthermore, analytical methods, such as high-pressure liquid chromatography (HPLC), can also detect the presence and quantity of peptides at very low concentrations, in a simple, fast, and efficient way, which allows the monitoring of conjugation reactions and its completion. Graphite/SiO2 film electrodes and HPLC methods were previously shown by our group to be efficient to detect drug molecules, such as losartan. We now use these methods to detect the conjugation efficiency of a peptide from the immunogenic region of myelin oligodendrocyte to a carrier, mannan. The HPLC method furthermore confirms the stability of the peptide with time in a simple one pot procedure. Our study provides a general method to monitor, sense and detect the presence of peptides by effectively confirming the conjugation efficiency. Such methods can be used when designing conjugates as potential immunotherapeutics in the treatment of diseases, including multiple sclerosis.
Highlights
Voltammetric techniques, including differential pulse voltammetry (DPV) and cyclic voltammetry (CV), as well as high-performance liquid chromatography (HPLC), were applied to identify and detect a peptide to its conjugated carrier
We We developed and confirm electrochemical method for monitoring the conjugation reaction of peptides to the carrier mannan; (KG)5-MOG35-55 was used as the peptide example in this reaction of peptides to the carrier mannan; (KG)5 -MOG35-55 was used as the peptide example in this study
Peptide-oxidized form (OM) conjugates can serve as potential vaccine candidates as has previously been study
Summary
Voltammetric techniques, including differential pulse voltammetry (DPV) and cyclic voltammetry (CV), as well as high-performance liquid chromatography (HPLC), were applied to identify and detect a peptide to its conjugated carrier. The performance of the voltammetric techniques is strongly dependent on the performance of the working electrode used Film electrodes, such as the graphite/SiO2 used in this study, are being used in electrochemistry, as it has a number of advantages over the standard metallic and glass carbon electrodes. These include ease of manufacture requiring lower temperatures, low cost, the high surface area that could be rapidly renovated, simple handling, Brain Sci. 2020, 10, 577; doi:10.3390/brainsci10090577 www.mdpi.com/journal/brainsci. These techniques exhibit a wide range of anodic and cathodic peaks and great electrocatalytic activity and stability All these features are crucial for the correct choice of a working electrode, especially when direct electrochemistry is conducted [1]. The peptide used was the multiple sclerosis (MS) immunogenic peptide from myelin oligodendrocyte (MOG35-55 )
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