The use anesthetic agents to promote welfare in anemonefish aquaculture: Case study to handling and long-distance transport

Abstract

Event Abstract Back to Event The use anesthetic agents to promote welfare in anemonefish aquaculture: Case study to handling and long-distance transport João Chambel1*, Marta Jacinto1, Rui Albuquerque1, Vera Severiano1, Susana L. Mendes1, Teresa Baptista1 and Rui Pedrosa1 1 School of Tourism and Maritime Technology, Marine Resources Research Group, Polytechnic Institute of Leiria, Portugal During their life cycle as farmed animals, there are several situations in which fish are subjected to handling and confinement. Netting, weighing, sorting, vaccination, transport and, at the end, slaughter are frequent events under farming conditions. The aim of this study was to evaluate the efficacy of three anaesthetic agents (80 to 110 mg/L of MS-222, 10 to 30 mg/L of clove oil and 100 to 350 mg/L of 2-phenoxyethanol) in anaesthesia and the success of use different clove oil concentrations (1, 3 and 10 mg/L) on water quality and survival of an ornamental anemonefish, Amphiprion percula, during long-distance transport in closed system for 48 hours. The lowest effective concentrations based on the efficacy criteria of complete anaesthetic induction within 180s, recovery within 300s and survival when exposed 30 min to anaesthetic was 15 mg/l for clove oil, 200 µl/L for 2-phenoxyethanol and 85 mg/L for MS-222. Above the concentrations of 30 mg/L, 300 µl/L and 100 mg/L of clove oil, 2-phenoxyethanol and MS-222 respectively, fish did not recovery when exposed to anaesthetic during 30min. During long-distance transport, clove oil slowed the increase on the ammonia concentration and the decrease on oxygen level, but did not affect the pH and only at concentration of 10 mg/L increase de mortality at 36 and 48 hours after packed. All concentrations showed a significance increase on water quality, reducing total ammonia from 9.31 ± 0.58 mg/L (control group) to 5.43 ± 0.56 mg/L (1mg/L), 4.97 ± 0.59 mg/L (3mg/L) and 3.88 ± 0.14 mg/L (10mg/L). This study showed that all anaesthetic agents under study can be used in anaesthesia of percula clownfish with optimal doses varied according to the anaesthetic, 15 to 25 mg/L of clove oil, 200 to 250 of 2-phenoxyethanol and 85 to 90 mg/L of MS-222, but clove oil was the anaesthetic agent more effectiveness and with major margin of safety for percula clownfish, and this anaesthesic (clove oil) can be used in ornamental fish transportation, reducing the deterioration in water quality and ensuring a better transport environment and reduced stress for the fish. However must be careful related the dose in use, because an excess anesthesic may reduce dissolved oxygen and cause high mortalities. Keywords: Fish anaesthesia, ornamental fish, Amphiprion percula, Anemonefish aquaculture, Clove Oil Conference: IMMR | International Meeting on Marine Research 2014, Peniche, Portugal, 10 Jul - 11 Jul, 2014. Presentation Type: Poster Presentation Topic: AQUACULTURE Citation: Chambel J, Jacinto M, Albuquerque R, Severiano V, Mendes SL, Baptista T and Pedrosa R (2014). The use anesthetic agents to promote welfare in anemonefish aquaculture: Case study to handling and long-distance transport. Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2014. doi: 10.3389/conf.fmars.2014.02.00128 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 May 2014; Published Online: 18 Jul 2014. * Correspondence: Mr. João Chambel, School of Tourism and Maritime Technology, Marine Resources Research Group, Polytechnic Institute of Leiria, Peniche, Portugal, joao.chambel@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers João Chambel Marta Jacinto Rui Albuquerque Vera Severiano Susana L Mendes Teresa Baptista Rui Pedrosa Google João Chambel Marta Jacinto Rui Albuquerque Vera Severiano Susana L Mendes Teresa Baptista Rui Pedrosa Google Scholar João Chambel Marta Jacinto Rui Albuquerque Vera Severiano Susana L Mendes Teresa Baptista Rui Pedrosa PubMed João Chambel Marta Jacinto Rui Albuquerque Vera Severiano Susana L Mendes Teresa Baptista Rui Pedrosa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.