Abstract
Azo dye techniques were used to investigate the ultrastructural localization of lysosomal acid hydrolases in ovarian oocytes of the common marine mussel Mytilus edulis. The enzymes were arylsulphatase, beta-glucuronidase, nonspecific esterase, N-acetyl-beta-hexosaminidase and acid phosphatase. For arylsulphatase, the azo dye technique was compared with an alternative method using nitrocatechol sulphate as the substrate and barium as the capturing ion. Activity of all the enzymes was found to be associated with the yolk granules and with pinocytotic phenomena which were observed along the basal membrane of developing oocytes. Activity was also found to be associated with resorption of atretic oocytes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.