Abstract
The distribution of acid and alkaline phosphatase was studied throughout sequential tooth development in the mouse. The azo dye and osmium-capture techniques were shown to provide comparable enzyme localization. Acid phosphatase was found mainly in the distal cytoplasm of functional cell body and process of both ameloblasts and odontoblasts. Alkaline phosphatase activity was observed in the prefunctional and active odontoblasts. In ameloblasts, positive activity was observed only after some enamel mineralization had occurred and was concomitant with a reduction in cell length.
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