Abstract
In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice ( Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) ( OsHK1–4, OsHKL1), 5 HPs (histidine phosphotransfer proteins) ( OsHP1–5), 15 type-A RRs (response regulators) ( OsRR1–15), 7 type B RR genes ( OsRR16–22), and 5 predicted pseudo-response regulators ( OsPRR1–5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway.
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