Abstract
The studThe Tolerance of Lactobacillusy conducted to obtain Lactobacillus species from bovine colostrum as probiotic candidate that have resistant character towards acidity and bile salt condition. Research done experimentally with Factorial Completely Randomized Design Pattern. The results were analyzed using Analysis of Variance (ANOVA) followed by Duncan's Multiple Range Test (DMRT). Results showed that L. paracasei and L. curvatus have tolerance of pH 2 with colony population by 0.07102 CFU/mL. L. paracasei and L. curvatus also have tolerance as well towards 0.3% of bile salt concentration with colony population by 4.051010 CFU/mL and 0.5% of bile salt concentration with colony population by 4.021010 CFU/ml. L. paracasei and L. curvatus have character as probiotic because grew well at high acidity and bile salts condition.
Highlights
Probiotic bacteria are non-pathogenic microorganisms that can control microbial populations in the human digestive system and has a benefit to its host
The competition mechanisms that can control population of pathogenic bacteria in the digestive system provided by probiotic bacteria (De Smet et al, 1995; Bezkorovainy, 2006)
Acclimatization or optimization needed if L. paracasei and L. curvatus originated from bovine colostrum will used as probiotic, so that the population of bacteria can meet the standards
Summary
Probiotic bacteria are non-pathogenic microorganisms that can control microbial populations in the human digestive system and has a benefit to its host. These bacteria have the criteria for probiotic bacteria such as viability at high acidity and bile salt tolerance (Kusumawati, 2002). Originated from bovine colostrum that have character of acidity and bile salts tolerance so it can be used as probiotic bacteria.
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