Abstract
Angelicae pubescentis radix (APR) is widely applied in treating rheumatoid arthritis in China. Coumarins are the major active compounds of APR extract including columbianetin, columbianetin acetate, osthole, and columbianadin. The in vivo behavior of the four major coumarins of APR has not been systematically reported. A feasible and reliable ultra-performance liquid chromatography (UPLC) method was established and validated for the quantification of the above four coumarins in rat various tissues (including heart, liver, spleen, lung, kidney, uterus, ovary, and muscle) after oral administration of APR extract. The separation was implemented on a Waters ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7 μm) with gradient mobile phase comprising acetonitrile-water (with 1mM formic acid) at a flow rate of 0.3 mL/min. The tissue homogenate samples were prepared by liquid-liquid extraction with ethyl acetate. The calibration curves were linear in the range of 1.6-20000 ng/mL for four coumarins with the lower limit of quantitation of 1.6 ng/mL in rat tissues. The intraday and interday precisions and recoveries were all within 80-100% with the relative standard deviations (RSDs) which were all less than 10.9%. The method was successfully applied to the tissue distribution research after oral administration of 6.0 g/kg APR extract to rat. The results revealed that the tissues distributions of four coumarins were in the liver, followed by the ovary, uterus, kidney, lung, heart, spleen, and muscle.
Highlights
Traditional Chinese Medicine (TCM) has been crucial to prevent and treat diseases
The results revealed that the retention time of columbianetin, columbianetin acetate, osthole, and columbianadin was 10.9, 19.0, 21.3, and 22.4 min, respectively
A sensitive, reliable, and fast method was established and validated for the simultaneous tissue distribution of osthole, columbianetin, columbianetin acetate, and columbianadin after oral administration of Angelicae pubescentis radix (APR) extract in rat
Summary
Traditional Chinese Medicine (TCM) has been crucial to prevent and treat diseases. TCM was applied to treat the illness depending on the theory of Traditional Chinese Medicine, which contains viscera-state theory [1]. According to the cure rules of chemical drugs, the tissues are the functional unit which maintains the homeostasis. In consideration of the function level, the viscera-state theory has similar effect with tissue level restoration in treating diseases. It can be seen that the tissue distribution study might act on the elucidation of healing mechanism of TCM. Angelicae pubescentis radix (APR) originates in the root of Angelica pubescens Maxim. APR possesses the efficacy of dispelling wind and removing obstruction in the meridians, activating blood and relaxation tendons, dispersing cold, and relieving pain [3], especially
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Evidence-based complementary and alternative medicine : eCAM
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
