The T786C polymorphism of NOS3 gene effect on the level of nitric oxide metabolites among patients with non-valvular paroxysmal atrial fibrillation

Abstract

The objective of the research - to determine the the T786C polymorphism of nos3 gene effect on the level of nitric oxide metabolites among patients with non-valvular paroxysmal atrial fibrillation.Material and methods. To achieve this goal, a prospective study was conducted on the basis of the communal uncommercial company “City Hospital № 10” of Zaporizhzhia City Council. The sample of patients was conducted in the period from 2014 to 2019. The results of the study are based on data from a comprehensive examination and dynamic monitoring of 176 patients with paroxysmal atrial fibrillation on the background of coronary heart disease combined with hypertension, of which 98 were from the city of Zaporizhzhia and 78 were from rural areas. Almost healthy 31 volunteers were examined on an outpatient basis.Results. Plasma NO2 levels in both groups area urban and rural patients - 7.02 [5.74 ; 8.14] mmol/L and 6.77 [5.47 ; 7.98] mmol/l, respectively, were significantly lower compared to 8.46 [7.45 ; 9.45] mmol/L in the healthy group (p<0.05). Significantly, the lowest level of NO3 was in the group of patients from the city of 10.36 [8.14 ; 13.32] mmol/L, in vs. to the value of 11.69 [10.36; 15.33] mmol/L in the group of patients from rural areas and against 13.36 [11.85 ; 15.35] mmol/L in the group of healthy individuals, (p<0.05). The highest value of NO2+NO3 was in the group of healthy people - 21.56 [20.38 ; 24.53] mmol/L, against the level of 18.02 [15.83 ; 21.78] mmol/L in the group of patients from rural areas and - 17.60 [14.80 ; 20.35] mmol/L in the group from urban area (p < 0.05). The median NO2 level in the subgroup of homozygotes for the T allele was 7.86 [6.66; 8.88] mmol/L and was significantly higher than in the subgroup of heterozygotes TC - 6.66 [5.18; 7.98] mmol/L – and the subgroup of homozygotes for the C - 5.81 [5.07; 6.72] mmol/l (p<0.05). The highest value of NO2+NO3 was in the homozygote subgroup for the T - 20.35 [17.07 ; 25.16] mmol/l, both against the level of 17.52 [15.37 ; 20.72] mmol/L of the TC heterozygote subgroup, and against 16.41 [14.48; 17.93] mmol/L of the homozygote subgroup for the C allele (p<0.05). However, there was no significant difference between the heterozygote and homozygote subgroups for the C allele (p>0.05). Conclusions. Decreased nitric oxide metabolites occur among patients with non-valvular paroxysmal atrial fibrillation compared with the healthy group. The level of nitrate-ions in the group of patients from the city was significantly lower than in the group of patients from the rural areas. The level of nitrite-ions was lower among patients with the C allele of the T786C polymorphism of the 3 type of nitric oxide synthase gene, whereas the value of nitrate-ions did not depend on this polymorphism.