Abstract
This paper presents a synthesis of a novel nanoparticle label with selective biorecognitionproperties based on a biotinylated silver-dendrimer nanocomposite (AgDNC). Two types oflabels, a biotin-AgDNC (bio-AgDNC) and a biotinylated AgDNC with a poly(ethylene)glycolspacer (bio-PEG-AgDNC), were synthesized from a generation 7 (G7) hydroxyl-terminatedethylenediamine-core-type (2-carbon core) PAMAM dendrimer (DDM) by anN,N′-dicyclohexylcarbodiimide (DDC) biotin coupling and aNaBH4 silver reduction method. Synthesized conjugates were characterized by several analyticalmethods, such as UV–vis, FTIR, AFM, TEM, ELISA, HABA assay and SPR. Theresults show that stable biotinylated nanocomposites can be formed either withinternalized silver nanoparticles (AgNPs) in a DMM polymer backbone (‘typeI’) or as externally protected (‘type E’), depending on the molar ratio of thesilver/DMM conjugate and type of conjugate. Furthermore, the selective biorecognition function ofthe biotin is not affected by the AgNPs’ synthesis step, which allows a potentialapplication of silver nanocomposite conjugates as biospecific labels in variousbioanalytical assays, or potentially as fluorescence cell biomarkers. An exploitation of thepresented label in the development of electrochemical immunosensors is anticipated.
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