Abstract
1,25(OH) 2D 3 and TGF-β can influence the function and differentiation of dental pulp fibroblasts. In this study, we examined the effect of 1,25(OH) 2D 3 and TGF-β on the synthesis of SPARC and ALP activity in human pulp fibroblasts. Two isoforms of SPARC, the 43 and 38 kDa, were detected in this cell type. TGF-β increased the synthesis of SPARC about 2.5-fold after 3 days of treatment but had no effect on the ALP activity. On the contrary, 1,25(OH) 2D 3 increased ALP activity 2-fold but had no effect on SPARC. The combination of TGF-β and 1,25(OH) 2D 3 significantly induced SPARC synthesis and ALP activity by 5 and 9 folds, respectively ( P<0.05). This finding suggested the synergistic effect between TGF-β and 1,25(OH) 2D 3 in dental pulp fibroblasts on the synthesis of SPARC and ALP activity. This interaction could influence the function and differentiation of dental pulp fibroblasts.
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