Abstract
Objectives: The aim of this study was to compare the cytotoxic effect of a methacrylate-based and a silorane-based composite on the human dental pulp stem cells (DPSCs) versus human dental pulp fibroblasts (DPFs). Study Design: Samples of the Filtek Z250 and P90 were polymerized and immersed in the culture medium to obtain extracts after incubation for one, seven and 14 days. Magnetic cell sorting based on the CD146 expression was performed to purify DPSCs and DPFs. After incubation of both cells with the extracts, cytotoxicity was determined using the MTT test. Results: For the extracts of first and seventh day, both composites showed significantly lower cytotoxicity on DPSCs than DPFs (p=0.003). In addition, there was a significant difference in the time-group interaction of both materials indicating different cytotoxic behaviours (p=0.014). In contrast to Z250, exposure to the 14th day extract of P90 resulted in higher cell viability compared to that of day seven. Conclusions: DPSCs are less susceptible to the cytotoxic effect of the composites than DPFs. Compared to Z250, the cytotoxic effect of silorane-based composite decreases as the time passes on. This difference should be considered, particularly in deep cavities, in order to preserve the regenerative capacity of the pulp. Key words:Composite resins, Dental pulp, Mesenchymal Stromal Cells, Silorane, Toxicology.
Highlights
Composite resin materials are widely used in various applications in routine dental restorative procedures
Many in vitro studies have shown that substances released from the composite resins due to resin degradation or incomplete polymerization can diffuse through dentin and reach the pulp tissue [5]
The aim of this study is to investigate the viability of the dental pulp stem cells (DPSCs) and dental pulp derived fibroblasts (DPFs), as terminally differentiated cells, after exposure to methacrylate and silorane-based composites
Summary
Composite resin materials are widely used in various applications in routine dental restorative procedures. Siloxane imparts the hydrophobic properties of silorane monomers while oxirane is responsible for its low shrinkage during polymerization. The mechanism of this polymerization relies on the ring opening cationic reactions [3]. Many in vitro studies have shown that substances released from the composite resins due to resin degradation or incomplete polymerization can diffuse through dentin and reach the pulp tissue [5]. These substances are able to affect the vitality and regenerative capacities of the pulp [6]. The resultant odontoblasts layer destruction leads to proliferation and migration of stem cells to the injury site and their differentiation into odontoblast-like cells
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