Abstract
Light-sheet Fluorescence microscopy (LSFM) with scanned Bessel beam draws a strong attention because it provides certain advantages. Especially, a thin light-sheet over a large field can be produced by Bessel beams. Also, as a non-diffracting beam, the Bessel beam enables deeper penetration into thick cells or tissues. Usually, the Bessel beam with high side-lobes is created by inserting an annular pupil filter in a Gaussian beam. The fluorescence background from the side lobes of the Bessel beam blurs the image and broadens the thickness of the light-sheet, which results in a lower image contrast and sacrificing the optical sectioning capability. To address this issue, in this paper, a leaky filter is designed to be inserted in the Gaussian beam to create a similar Bessel beam with lower side lobes. By optimizing the parameters of the leaky filter, the suppression of the side lobes is demonstrated.
Published Version
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