The suitability of biotypes Q and B of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) at different nymphal instars as hosts for Encarsia formosa Gahan (Hymenoptera: Aphelinidae).

Xin Liu,Wen Xie,Shaoli Wang,Qingjun Wu,Youjun Zhang

doi:10.7717/peerj.1863

Abstract

Encarsia formosa Gahan (Hymenoptera: Aphelinidae) is a solitary endoparasitoid that is commercially reared and released for augmentative biological control of whiteflies infesting greenhouse crops. In most areas in China, the invasive and destructive whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype Q has replaced B. tabaci biotype B and has become dominant between the two. A better understanding of the suitability of different nymphal instars of B. tabaci biotypes Q and B as hosts for E. formosa is needed to improve the use of this parasitoid for biological control. Parasitism of the four nymphal instars of B. tabaci biotypes Q and B by the commercial strain of E. formosa mass reared on Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) was assessed in the laboratory. The results indicated that E. formosa parasitized and successfully developed on all instars of both biotypes but performed best on the 3rd instar of B. tabaci biotype B and on the 2nd, 3rd, and 4th instars of B. tabaci biotype Q. The host-feeding rate of the adult parasitoid was generally higher on nymphal instars of B. tabaci biotype Q than on the corresponding nymphal instars of biotype B and was significantly higher on the 2nd and 3rd instars. For both whitefly biotypes, the parasitoid’s immature developmental period was the longest on the 1st instar, intermediate on the 2nd and 3rd instars, and the shortest on the 4th instar. The parasitoid emergence rate was significantly lower on the 1st instar than on the other three instars and did not significantly differ between B. tabaci biotype B and biotype Q. Offspring longevity was greater on the 3rd and 4th instars than on the 1st instar and did not significantly differ between the two B. tabaci biotypes. The results indicate that commercially-produced E. formosa can parasitize all instars of B. tabaci biotypes B and Q, making this parasitoid a promising tool for the management of the two biotypes of B. tabaci present in China.

Highlights

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a serious pest of many important vegetable, field, and ornamental crops in the tropics and subtropics
Parasitism rate of Encarsia formosa as affected by Bemisia tabaci biotype and instar The parasitism rate of E. formosa was significantly affected by host instar (F3,151 = 22.43, P < 0.0001) but not by host biotype (F1,151 = 1.65, P = 0.200)
Differences in the host suitability in terms of initial parasitism, host-feeding, or parasitoid development may result from differences in host quality, which may vary with host age (Colinet et al, 2005)

Summary

Introduction

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a serious pest of many important vegetable, field, and ornamental crops in the tropics and subtropics. The two most invasive and destructive species are the Middle East-Asia Minor I species and the Mediterranean species, formerly referred to as biotype B and Q, respectively (De Barro et al, 2011; Pan et al, 2012). Studies indicate that biotype Q has quickly replaced biotype B and has become the dominate B. tabaci biotype in many regions of China (Chu et al, 2010; Teng, Wan & Chu, 2010; Pan et al, 2011). Biotype Q is currently considered a more serious pest than biotype B because of its greater virus transmission efficiency (Pan et al, 2012; Liu et al, 2013) and higher insecticide resistance (Rauch & Nauen, 2003; Luo et al, 2010; Xie et al, 2014)

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