The study on the effect of mercury pollution on soil microorganisms around mercury mining area

Abstract

In order to further explore the effects of soil mercury pollution on soil microbial diversity and community structure, soil samples were randomly collected from 2 m, 20 m, 30 m, 500 m and 650 m periphery of Wanshan mining area, as 5 different treatments. Each treatment had 4 replicates. Soil microbial DNA was extracted from 20 soil samples, and then high-throughput sequencing technology was used to analyse the structure and distribution of bacterial and fungal communities. The results showed that the number of bacterial and fungal communities in T0–T30 treatments was significantly larger than that in T500–T650 treatments at order, family and genus level. Whatever, the number of uniquely distributed bacterial and fungal communities among 4 replicates soil samples was quite different at order, family and genus level. The results of the effect on the microbial community structure showed that there were both the same dominant bacterial and fungal communities, and the different dominant bacterial and fungal communities at any classification level, moreover, the number of same dominant bacterial and fungal communities was larger than that of different dominant bacterial and fungal communities. The results of relationship between soil environment factors and bacterial and fungal community structure showed that distance (Hg2+), EC and pH had a high correlation with community structure, especially the distance factor, that is, the content of mercury in soil had the highest effects on community structure. The internal heterogeneity of soil caused significant differences in bacterial and fungal community structure, and the emergence of dominant bacterial and fungal communities was a manifestation of better adaptability to long-term mercury stress and other stresses in soil, which will provide a scientific reference for further exploring the mechanism of mercury enrichment between microorganisms and plants.