The Study of The Interaction Between Parkin and OGCP*

Abstract

Parkin gene is one of the virulence gene in Parkinson’s disease. Being an E3 enzyme of ubiquitin-proteasome pathway(UPP), Parkin protein mediates many substrate proteins’ubiquitination which has been closely linked to the pathogenesis of Parkinson’s disease. 2-oxoglutarate carrier protein (OGCP) is a mitochondrial membrane protein. Immunofluorescence and laser confocal microscope confirmed that in HEK293 cells, there are co-orientation and interaction between Parkin protein and OGCP. And Parkin protein can mediate the ubiquitination of OGCP though in vitro and in vivo ubiquitination essays. These findings suggest that OGCP may be one of the ubiquitination substrate proteins of Parkin protein and Parkin protein mediates can promote the OGCP’s ubiquitination.