The study of the C-X-C chemokine receptor 4 expression on monocyte-derived macrophage from active tuberculosis patients

Abstract

Objective To study the expression of C-X-C chemokine receptor 4 on monocyte-derived macrophage (MDM) from active tuberculosis patients. Methods Patients with active pulmonary tuberculosis and healthy subjects were recruited from 309 hospital.Monocytes were isolated from peripheral blood and cultured in vitro for differentiation to M0 type macrophages.Macrophages were then activated with lipopolysaccharides (LPS)/ interferon gamma (IFN-γ) or IL-4 for 24 hours to polarize to M1 type macrophages or M2 type macrophages.Total RNA was extracted from macrophages.Fluorescence quantitative polymerase chain reaction (PCR) was used to detect the mRNA expression of CXCR4, and differences of CXCR4 mRNA between two groups were analyzed by t test statistic method. Results The expression of CXCR4 mRNA in M1 type MDM from patients with active pulmonary tuberculosis was significantly lower than that of healthy controls (0.026±0.009 vs 0.034±0.014, t=2.064, P<0.05). Similarly, the expression of CXCR4 mRNA in M2 type MDM from active tuberculosis patients was significantly lower than that of healthy control (0.048±0.013 vs 0.064±0.019, t=2.823, P<0.01). Conclusions The expression of CXCR4 in M1 and M2 type MDM from patients with active patients was decreased, suggesting that the CXCR4 of macrophages participates in the anti-tuberculosis immunity. Key words: Tuberculosis, active; C-X-C chemokine receptor 4; Monocyte-derived macrophage; Polarization of macrophage