The research of polarization of monocyte-derived macrophages from patients with active tuberculosis

Abstract

Objective To study the polarization of monocyte-derived macrophages (MDMs) to the M1 and M2 macrophages, which were obtained from active pulmonary tuberculosis patients. Methods Peripheral bleed were collected from patients with active pulmonary tuberculosis and healthy people.Peripheral blood mononuclear cells (PBMCs) were separated from peripheral bleed.CD14+ mononuclear cells were purified from PBMCs by magnetic activated cell sorting (MACS) and in vitro differentiated to M0 macrophages.Bacterial lipid polysaccharide (LPS)/interferon-γ (IFN-γ) and interleukin-4 (IL-4) were added to the culture of M0 macrophages and were used to stimulate the M0 macrophages to M1 and M2 macrophages, Flow cytometry method was used to detect the markers on/in M1 and M2 macrophages.The markers of CD80, CD86 and HLA-DR were mainly expressed on/in M1 macrophages and the markers of CD163 and CD206 were mainly expressed on/in M2 macrophages. Results Compared with healthy people, CD80 and CD86 expressed in M1 macrophages collected from patients with active pulmonary tuberculosis were significantly decreased (t=2.359, P<0.05; t=2.816, P<0.01), and CD163 and cd206 expressed in M2 macrophages surface were significantly increased (t=2.368, P<0.05; t=2.994, P<0.01). Conclusions Polarization ability of MDM to M1 macrophages is decreased and to M2 macrophages are enhanced, which were collected from patients with active pulmonary tuberculosis. Key words: Pulmonary tuberculosis, active; Monocyte derived macrophage; Polanzation