The study of the antimicrobial activity of ointments with the lipophilic extract from Salix viminalis L. Shoots

Abstract

The increase in the number of purulent-inflammatory diseases and postoperative purulent complications, as well as deterioration of the results of treating purulent surgical infections, including the formation of antibiotic-resistant strains of microorganisms, leads to the search for effective antimicrobial substances. Plant extracts can be used for these purposes.Aim. To expand the range of domestic ointments containing substances of natural origin as active pharmaceutical ingredients (API). To achieve the aim the team of authors has studied the possibility of developing an ointment on a hydrophilic base using the lipophilic extract from Salix viminalis shoots as API and the analysis of its antimicrobial properties.Materials and methods. As the study object the samples of semisolid medicinal forms containing the lipophilic extract from Salix viminalis shoots in the concentration from 1 % to 10 % on a hydrophilic ointment base with Tween-80, propylene glycol (PG) solution and an alloy of polyethylene oxides (PEO) 400 and 1500 were used. As the method of the samples efficiency control the method of diffusion into agar with the growth inhibition zone determination was chosen. Staphylococcus aureus ATCC 25923, Esherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis ATCC 6633, Proteus vulgaris ATCC 4636 and Candida albicans ATCC 885-653 strains were used.Results and discussion. The studies conducted have proven the presence of the antimicrobial activity in the samples of ointments on a complex hydrophilic base. A gradual increase in the antimicrobial activity of ointments has been found with an increase in the concentration of the extract from Salix viminalis shoots. The results obtained can be used as the base for further biopharmaceutical research and the aim implementation.Conclusions. As a result of the studies conducted the optimal concentration of the Salix viminalis extract in the ointment composition on the hydrophilic base has been determined. The composition developed exhibits a pronounced antimicrobial activity against strains of Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Basillus subtilis and Candida albicans. The ointment can be used to treat infectious complications of wounds.