The study of effects of the artificial synthetic E-selectin on proliferation activity of smooth muscle cell in intracranial aneurysm wall of common carotid artery aneurysm in rabbits

Abstract

Objective To investigate the effects of artificial synthetic E-selectin on the proliferation activity of smooth muscle ceils in the intracranial aneurysm wall of common carotid artery aneurysms in rabbits.Methods 54 healthy New Zealand rabbits,half male and half female,were randomly divided into nine groups(each group =6):Control groups,1,2,3 and 4 weeks experiment groups,1,2,3,and 4 weeks treatment groups.The morphologic change of the right common carotid artery and formation of the aneurysm were detected by CT angiography and HE dyeing.The aneurysm samples were collected,the protein of MMP-2、PCNA,and α-SMA were detected by immnunohistochemistry,and the mRNA expression of OPN and MMP-2 were detected with immunohistochemistry and real-time polymerase chain reaction.Results Compared with the experimental groups,the width and altitude of the aneurysms in the treatment groups decreased.The immunohistochemistry results disclosed that there were different level reductions of MMP-2 expression between the experimental groups and the treatment groups at each time point.The expression of PCNA of experiment groups decreased compared with treatment groups respectively,especially at the 1 week point.There were different level reductions of α-SMA expression between experimental groups and treatment groups at each time point.Real-time PCR results:the Real-time PCR detection outcome marked that there were different level increases of OPN expression between experimental groups and treatment groups at each time point,while there were also different level reductions of MMP-2 expression between experimental groups and treatment groups at each time point.Conclusions The artificial synthetic E-selectin can inhibit the reduction of the quantity of smooth muscle cells and smooth muscle cells layers ; and it can promote the transformation of smooth muscle cells to synthetic phenotype,strengthen the proliferation activity of smooth muscle cells,and then repair the aneurysm wall. Key words: Aneurysm; Model; Vascular smooth muscle cells; Proliferation; Artificial synthetic E-selectin