The study of antioxidants in grapevine seeds

Mojmí­r Baroň,Lenka Tomášková,Jiří­ Sochor

doi:10.5219/711

Abstract

Grapevine seeds contain a large amount of antioxidant components, and are therefore recommended in the prevention and treatment of many diseases. For this research, we studied the antioxidant properties of grapevine seeds from the Marlen variety, as evidence suggests that these types have higher resistance against fungal diseases. Through high-performance liquid chromatography with UV/VIS detection, a total of 10 antioxidant components were selected for further investigation, specifically: catechin, epicatechin, rutin, quercitrin, quercetin, caftaric acid, caffeic acid, p-coumaric acid, ferulic acid, and gallic acid. The antioxidant activity was determinated spectrophotometrically through the adoption of three fundamentally different methods (the DPPH assay, the ABTS method, and the FRAP method). Using the Folin-Ciocalteu method, it was possible to determine the content of all the polyphenolic compounds. The results of the assessment antioxidant activity and the content of polyphenolic compounds were recalculated to gallic acid equivalents (GAE). The values of the antioxidant activity as determinated by the DPPH test were 6643 (±154) mg of GAE; 1984 (±88) mg of GAE when using the FRAP method; and 812 (±31) mg of GAE when the ABTS method was utilised. The content of the total polyphenolic compounds came to 6982 (±221) mg of GAE. The most abundant antioxidant was catechin, with a content of 115 mg.L-1, whilst the least represented compound was ferulic acid (0.139 mg.L-1). Overall, this study showed a high antioxidant potential of grapevine seeds.

Highlights

Antioxidants are potent scavengers of free radicals and serve as inhibitors of neoplastic processes (Bagchi et al, 2000)
Our work focuses on the study of antioxidant activity and polyfenolic compounds in grapevine seeds, with the main target being to determine 10 antioxidant components through high performance liquid chromatography
A wide range of methods to determine antioxidant activity can be found in existing literature, highlighting how low molecular weight antioxidants act differently depending on the mechanism used

Summary

Introduction

Antioxidants are potent scavengers of free radicals and serve as inhibitors of neoplastic processes (Bagchi et al, 2000). Grape seeds are rich sources of polyphenolic compounds, which are characterised by a variety of properties, such as antibacterial and antioxidant activities (Berradre et al, 2013). Results of many studies indicate that grapevine seeds are a source of biologically active substances usable for pharmaceutical and other purposes (Ali et al, 2010). Various research has indicated that the phenolics in grapes can be used to prevent atherosclerosis (Pekić et al, 1998). Recognition of the health benefits of catechins and procyanidins has led to the use of grape seed extract as a dietary supplement (Fuleki & Ricardo da Silva, 1997). The naturally occurring antioxidant of oligomeric proanthocyanidins has been reported to possess a broad spectrum of therapeutic benefits (Bagchi et al, 2000). The antioxidant activity in grapevine seeds has been studied across a number of popular and hybrid varieties; further studies should start focusing more on interspecific varieties, such as Marlen, to determine their antioxidant activity and phenolic content (Yilmaz et al, 2015)

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