The Study of Antibacterial Activity of Juglans Regia and Thymus Vulgaris Seeds

Abstract

It has been well known since ancient times that medicinal plant have antimicrobial activity because of the presence of substances, therefore they become important sources of drugs production. Thymus vulgaris and Juglans regia have active compounds make them have antibacterial properties.Antibacterial effects of different concentrations ranging from (3.1- 50mg/ml) of hexane and methanol extracts of Thymus vulgaris and Juglans regia seeds was determined by using agar well diffusion method on clinical strains: Staphylococcus aureus, Listeria monocytogenes, Streptococcus, pyogenes, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Salmonella typhi Klebsiella pneumoniae and Enterococcus sp.Methanol extract of Juglans regia seed was the most active followed by methanol extract of Thymus vulgsris seeds. The activity of Juglans regia extractdetermined by measuring inhibition zone as following:Staphylococcus aureus, Proteus vulgaris, Listeria monocytogenes and Streptococcus pyogenes showed inhibition zone at concentration of 3.1-50 mg/ml. Bacillus subtilis and Klebsiella pneumoniae showed inhibition zone of 12.5-50 mg/ml. Pseudomonas aeruginosa showed sensitivity at concentrations of 25-50mg/ml. There was no inhibition zone for Escherichia coli, Salmonella.typhi and Enterococcus sp.Methanol extract of Thymus vulgaris showed activity at concentrations of 6.25-50mg/ml for Listeria monocytogenes, and Bacillus subtilis.Escherichia coli, Staphylococcus. aureus, Proteus vulgaris, Klebsiella pneumoniae and Streptococcus pyogenes showed sensitivity at concentration of 12.5–50 mg/ml. Pseudomonas aeruginosa sensitive at concentration of 50- 25mg/ml. There was no inhibition zone for Salmonella.typhi and Enterococcus sp. Hexane extracts of both plant seeds were less active then methanol extract against tested bacteria.These results support the notion that plant extracts may have a role as pharmaceutical and preservatives.