Abstract
The aim of this study was to investigate if the modulation of the tumor c-Ha-ras1 mRNA expression may be one of the mechanisms implicated in the effect of the dietary n-6 polyunsaturated lipids on experimental mammary carcinogenesis. Sprague-Dawley rats were treated with dimethylbenz(α)anthracene and fed a low-fat or high-fat corn oil diet following an initiation-promotion experimental design. Results showed a shorter latency and significantly higher incidence of affected animals, total tumor yield and total tumor volume in groups of rats fed the high-fat diet than in the control low-fat diet group. This stimulating effect on mammary carcinogenesis, which was produced above all on the promotion stage, did not result in a modification of the tumor c-Ha-ras1 mRNA levels. Moreover, c-Ha-ras1 was not amplified in these experimental mammary tumors. These results do not discard the possible implication of this gene, and suggest that dietary fat may act on c-Ha-ras1 at a different level, maybe on protein function.
Published Version
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