The state of the prooxidant-antioxidant system in cows with different clinical conditions during pregnancy

Abstract

The purpose of the research was to study the effect of gestational age on the state of the enzymatic link of antioxidant protection (AOP) of lipid peroxidation products (LPO) in cows with anemia syndrome and chronic systemic inflammation. Three (3) groups of cows were formed: group I (n=15) – healthy; group II (n=8) – with hypochromic microcytic anemia; group III (n=7) – with chronic systemic inflammation syndrome of low intensity. On days 150-160, 210-220 and 260-265 of gestation, blood samples were taken to study the indicators of LPO, AOP and endogenous intoxication. There were obtained the data on the peculiarities of the state of the LPO-AOP system in cows with various clinical states during gestation. It has been established that there is an inverse correlation between the period of gestation and catalase activity. A significant direct correlation of high strength with respect to the level of nitric oxide has also been revealed. In cows with anemia, less intense tendencies for an increase in nitric oxide have been noted, which may have an important role in the control of fetoplacental hemodynamics and saturation of the fetal blood with oxygen. In group I and II, the level of diene conjugates had a significant direct correlation of noticeable strength, but in group III, the binding strength increased and became high, which was reflected at the level of molecules of average molecular weight, detectable at a wavelength of 254 nm. Thus, with an increase in the duration of gestation, there is an increase in the LPO intensity, which is most pronounced in cows with a syndrome of chronic systemic inflammation of a low degree of intensity, as a result of which endogenous intoxication develops. At the same time, nitric oxide is less actively produced in cows with anemia that can lead to the development of fetoplacental insufficiency and hypoxic state of the fetus.