﻿The state of pro- and antioxidant systems in rats with DMH-induced colon carcinogenesis on the background of extracorporeal detoxification

Oksana Kachur,Dmytro Fira,Liudmyla Fira,Iryna Stechyshyn,Petro Lykhatskyі

doi:10.3897/pharmacia.68.e71840

Abstract

Aim: Cancer is one of the leading causes of death in the world. The aim of this research was to study the indices of pro- and antioxidant systems in rats with dimethylhydrazine (DMH)-induced colon carcinogenesis on the background of the enterosorbent AUT-M use. Materials and methods: The study was performed on 70 white male rats weighing 200–250 g. Adenocarcinoma of the colon was simulated by subcutaneous injection of the DMH (Sigma-Aldrich Chemie, Japan) at a dose of 7.2 mg/kg once a week during 7 months. Enterosorbent AUT-M was administered intragastrically daily for 21 days after simulation of carcinogenesis at a dose of 1 ml of suspension per 100 g of animal body weight. The state of the pro- and antioxidant systems was studied by the content of oxidative modification of proteins products (OMP), the activity of superoxide dismutase (SOD), catalase (CAT), contents of ceruloplasmin (CP) and reduced glutathione (GSH). Results: It was found that DMH-induced colon carcinogenesis in rats is accompanied by disorders in the antioxidant defense system and activation of free radical oxidation processes. Enterosorbent AUT-M provides a significant reduction in the content of OMP370 and OMP430 in both blood serum and liver homogenate of rats. Moreover, the use of enterosorbent AUT-M demonstrated a significant increase in the activity of SOD, CAT, content of GSH and a decrease in CP content in investigated tissues. Conclusion: The use of enterosorbent AUT-М demonstrated prominent potential suppression for oxidative stress and positive effect on antioxidant defense system in rats with DMH-induced colon carcinogenesis.

Highlights

Cancer is one of the leading causes of death in the world and colorectal cancer is one of the three most commonly diagnosed cancers (Siegel et al 2020; Sung et al.2020)
It was found that in animals with DMH-induced colon carcinogenesis at 1st month of the experiment in the blood serum and liver homogenate, the content of OMP370 increased by 2.3 times (p
Similar dynamics of aldehyde- and ketone derivatives of an alkaline character (OMP430) changing was found in blood serum and liver homogenate of rats during all experiment: at 1st month in blood serum this index significantly increased by 1.3 times, in the liver homogenate – by 1.5 times vs control group

Summary

Introduction

Cancer is one of the leading causes of death in the world and colorectal cancer is one of the three most commonly diagnosed cancers (Siegel et al 2020; Sung et al.2020). Besides an ageing population and dietary habits of high-income countries, unfavourable risk factors such as obesity, lack of physical exercise, smoking and chlamydial infections increase the risk of colorectal cancer Boiko et al 2019; Borel et al 2018; Dekker et al 2019). Active generation of ROS enhances the processes of free radical oxidation and leads to the development of oxidative stress (Marushchak et al 2016, 2019). Influence of enterosorption on the development of oxidative stress in cancer patients has exceptional importance (Shevchuk et al 2012)

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