The state of endotoxin-antiendotoxin system in males with disorders of reproductive function

Abstract

Objective was the research of the state of endotoxin-antiendotoxin system in males with different disorders of their ejaculate fertility.Materials and methods. The study involved 87 men aged between 20 and 45 years, who were divided into five groups. The control group consisted of healthy men without reproductive disorders; comparison group (second group) - men with children; 3-, 4-, 5th group – men who were in a childless marriage. Men 2, 3, 4th and 5th at the time of the study groups were detected infections, sexually transmitted infections and during the bacteriological examination of ejaculate – bacteriospermia caused by gram-positive and gram-flora. All the men had experienced a comprehensive study, including analysis of the sperm according to WHO guidelines, the determination of total concentration of endotoxin in the systemic circulation using the "Micro-LAL-Test" (Russian patent number 2,169,367), and evaluation of humoral antiendotoxic immunity by determining the titers of antibodies to glycolipid and common antigen by enterobacteria "SOIS-ELISA" (Russian patent number 2,011,993).Research results. The analysis of spermograms revealed four degrees of impaired fertilityof semen: the firstone was accompanied by the preservation of fertility with a tendency to decrease (second group); 2nd – reduced fertility (3rd group);3rd – a significant reduction in fertility (4th group) and fourth – lack of fertile properties of ejaculate (5th group). In thestudy of the state of endotoxin-antiendotoxin system we revealed that men with infertility experienced endotoxin aggression, which, depending on the severity of changes in the fertile properties of ejaculate, was acute (3rd group) or chronic (4th and 5th group).Conclusions. Presence of infections which are sexually did not have substantial influence on the state of fertility propertiesof sperm. This research shows that endotoxin aggression, and in particular chronic one, is the important link of pathogenesis of males infertility, and determination of the state of endotoxin-antiendotoxin system at patients with disorders of reproductive function is the necessary component part of complex examination that will allow to perfect diagnostics of male infertility and will be favorable to working out optimum schemes of treatment.