The stability of accumulated anthocyanin in suspension cultures of the parental line and high and low accumulating subclones of wild carrot

Abstract

Abhibition of the accumulation of anthocyanin but not of growth in wild carrot cultures was achieved using 2-amino-oxyphenylpropionic acid or phenylpropiolic acid. The concentration of inhibitor required to achieve this differential effect increased with increased culture density. By several additions of inhibitor at differential times, anthocyanin accumulation in the parental culture and in high-and low-acculmulating subclones could be completely inhibited. Under these conditions the level of anthocyanin in the cultures remained unchanged. These data show that the accumulated anthocyanin is not metabolized and that differences in the rate of degradation of anthocyanin does not account for the differences in anthocyanin accumulation by the subclones examined.