Regulatory effect of stems on sucrose-induced chlorophyll degradation and anthocyanin synthesis in Egeria densa leaves

Abstract

Detached green leaves of the aquatic plant Egeria densa showed chlorophyll degradation and turned red due to induced anthocyanin synthesis incubated in 0.1M sucrose under continuous light for 7-10days. If the leaves were placed in water, only chlorophyll degradation occurred and the detached leaves turned yellow. The levels of endogenous total carbohydrates increased in detached leaves cultured in the sucrose solution but only increased marginally in water. If the leaves were still attached to a piece of stem, with a node on either side of the single leaf whorl, then they did not accumulate anthocyanin in culture with 0.1M sucrose. These leaves showed a similar increase in total carbohydrates and degradation of chlorophyll as detached leaves. Attached leaves, in which the midrib had been cut in situ, showed localized accumulation of anthocyanin in the leaf tissue distal to the cut in the midrib when cultured in 0.1M sucrose. These results suggest that the stem plays a regulatory role in anthocyanin synthesis in attached leaves cultured in a sucrose solution but does not influence chlorophyll degradation or carbohydrate accumulation.