Abstract
The present study was designed to elucidate the character of the distribution of 2,4- and 2,6-di-tret-butylhydroxybenzenes (2,4-DTBHOB and 2,6-DTBHOB respectively) in the body of the warm-blooded animals (rats) following the administration of the three-fold LD50 dose into the stomach. Both 2,4-DTBHOB and 2,6-DTBHOB were extracted from the blood and the organs of the perished animals by means of two-fold incubation of the sampled tissues in ethyl acetate with the subsequent purification of the isolates by passing the extracts through a L 40/100 mcm silicagel column using hexane:dioxane (8.5:1.5) for 2,4-DTBHOB and hexane:dioxane (97.5:2.5) for 2,6-DTBHOB as eluants. The compounds of interest were identified and quantified by means of TLC, HPLC, and UV-spectrometry. The study has shown that both 2,4-DTBHOB and 2,6-DTBHOB were present in the organs and blood of the poisoned animals in the unmetabolized form. Their largest amounts (mg/100 g) were found in the contents of the stomach, the small intestines with the contents and in the spleen.
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