The Specific Binding between Aptamer and its Target Molecules Investigated by Microcantilever Sensor

Abstract

Cantilever with modified aptamers on the gold surface was employed to detect platelet-derived growth factor-BB (PDGF-BB) and cocaine. The deflection increased when PDGF-BB and cocaine were added into the flowing cell, respectively. The defection depended on the concentration of PDGF-BB and cocaine. It was also found the microcantiver went back to the same position as without cocaine after adding cocaine aptamer in situ, which proved that the cantilever could be reused. The deflection arose from the specific interaction between aptamer and its target.