Abstract
AbstractThe main objective of this study is to obtain some knowledge of cationic and anionic radicals in early embryo implantation in mice. The method used in this study is known as histochemical staining, in which Xylidine ponceau was used at pH 2.5 and toluidine blue was used at pH 4.0. We detected the change in glycosaminoglycans and total proteins in the endometrial stroma during the preimplantation of mice. This study revealed that the distribution patterns of cationic radicals and anionic radicals are similar on days 4 and 5 of pregnancy. However, there was a distinct difference between cationic radicals and anionic radicals on day 8 of pregnancy. The distribution pattern of cationic radicals is more concentrated in the stroma near the conceptus. Laboratory studies on histochemical stain provide more information about early embryo implantation.
Highlights
Implantation has been of considerable interest to the pregnancy community in recent years [1]
Slides were stained with toluidine blue (TB) and Xylidine ponceau (XP) for morphological evaluation
It is well known that NH3+ radical groups are present in total proteins when solution is at pH 2.5
Summary
Implantation has been of considerable interest to the pregnancy community in recent years [1]. Many protein signal molecules have undergone specific changes during the implantation process. Experiments have shown that there is a regular change in the endometrial surface sugar during implantation. Studies have shown that the presence or absence of blastocysts in the uterus has a significant effect on endometrial glycoprotein expression. The aforementioned results indicate that the changes of signal molecules during the implantation period are affected by hormones and related to the local effects of blastocysts. The previous study detected the cationic and anionic radicals using Xylidine ponceau (XP) at pH 2.5 and toluidine blue (TB) at pH 4.0 during the early phase of embryo implantation of rats [5]. The initial interaction between the concept and the uterus is a complex phenomenon, little research has been done on the spatial and temporal distribution of cationic radicals and anionic radicals during the time of implantation
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