Abstract
Implantation involves a complex process between the embryo and uterus and is crucial to further embryonic development. In this histochemical study, we investigated the early stage of embryo implantation and artificial decidualization in mice by the detection of total proteins and glycosaminoglycans using toluidine blue at pH 4.0 (TB) and Xylidine Ponceau at pH 2.5 (XP). TB staining showed mild metachromatic basophilia, which was more visible in the endometrial stroma around the implantation site in day 5 of pregnancy, and the level of glycosaminoglycans was more intense near the site of implantation on day 6, 8. Histochemical staining with XP was observed more intense in the stroma close to the site of implantation, especially in day 8 of pregnancy. At sites distant from the blastocyst were observed more discrete by staining with either TB or XP. Compared with TB, XP were more evident in the stroma around the uterine lumen under artificial decidualization. This study demonstrated changes in glycosaminoglycans and proteins by the detection of anionic and cationic radicals in the endometrial stroma adjacent to the site of embryo implantation during peri-implantation, post-implantation and artificial decidualization.
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