Abstract
Microsomes prepared from house fly abdomens were solubilized with sodium deoxycholate and resolved on a DEAE cellulose column into two fractions containing cytochrome P-450. On the basis of spectral characteristics the cytochrome in a fraction eluting with 0.3M KCl is different from that eluting with 0.5M KCl. The two forms of P-450 were found in both susceptible and resistant strains of house flies. The P-450 in the 0.5M KCl eluting fraction appears to be more unstable than that in the 0.3M fraction. These results are in disagreement with those of other workers who have concluded that only one of two possible forms of cytochrome P-450 is present in a given strain of the house fly.
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