The simultaneous multiple measurement system of serum opsonic activity using the technic of luminol-dependent chemiluminescence

Abstract

The method of luminol-dependent chemiluminescence (LC method) is one of the measurement methods of the neutrophilic phagocytosis, which has been reported to give results similar to other previous methods and has good reproducibility.We have succeeded in making a simultaneous multiple measurement system using the technique of LC method and also reported some basic findings on the measurement method of serum opsonic activity.1. To introduce a one-step separation procedure of neutrophil from whole blood, the lag time from drawing blood to starting measurement is shortened.2. A remodeled Lumiphotometer TD 4000 (Labo Science Co. ltd.) is used to measure the luminescence. Using a digitizer, each value of measured luminescence (represented in voltage) is fed into the computer. Data of individual samples is printed out in the form of figures; the starting point is the time of adding a neutrophil suspension into the opsonized particles suspension, a horizontal axis means the elapsed time and a vertical axis means the intensity of luminescence.3. The simultaneous multiple measurement system in this report also exhibits an excellent property of reproducibility. In the region of maximum luminescence, coefficient of variation is a mere 2 or 3% in all cases in this report.4. According to the results of the simultaneous measurement using a standard serum and same neutrophil, we recognize that the luminescence patterns are characteristic of the particle species.5. For the purpose of producing the samples graded by the activity of the thermolabile opsonic activator, inactivated serum (56°C, 30min) and normal serum are mixed in specified proportions. From the results of the simultaneous measurement of these samples of plural particles, we can assume that the heat-inactivation effect on the opsonic activity is different from the particle species.