Abstract
The goal of this study was to determine and compare the effects of the secretome of mesenchymal stem cells (MSCs) isolated from human bone-marrow (BMSCs) and the Wharton jelly surrounding the vein and arteries of the umbilical cord (human umbilical cord perivascular cells (HUCPVCs)) on the survival and differentiation of a human neuroblastoma cell line (SH-SY5Y). For this purpose, SH-SY5Y cells were differentiated with conditioned media (CM) from the MSCs populations referred above. Retinoic acid cultured cells were used as control for neuronal differentiated SH-SY5Y cells. SH-SY5Y cells viability assessment revealed that the secretome of BMSCs and HUCPVCs, in the form of CM, was able to induce their survival. Moreover, immunocytochemical experiments showed that CM from both MSCs was capable of inducing neuronal differentiation of SH-SY5Y cells. Finally, neurite lengths assessment and quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) analysis demonstrated that CM from BMSCs and HUCPVCs differently induced neurite outgrowth and mRNA levels of neuronal markers exhibited by SH-SY5Y cells. Overall, our results show that the secretome of both BMSCs and HUCPVCs was capable of supporting SH-SY5Y cells survival and promoting their differentiation towards a neuronal phenotype.
Highlights
Central nervous system (CNS) neurological disorders/injuries often pose a major challenge for treatment due to the limited capability of CNS to self-renew and to regenerate [1]
SH-SY5Y cells incubated with a combination of low percentage of FBS and retinoic acid (RA) treatment were used as positive control of SH-SY5Y cells differentiation (RA-differentiated cells)
Results revealed that cells incubated with both BMSCs and HUCPVCs conditioned media (CM) had similar levels of metabolic viability after 7 days of culture (Figure 1)
Summary
Central nervous system (CNS) neurological disorders/injuries often pose a major challenge for treatment due to the limited capability of CNS to self-renew and to regenerate [1]. These CNS features have prompted the search for new therapies, such as those using mesenchymal stem cells (MSCs). MSCs have been defined as multipotent cells which are capable of self-renewal [2]. Robust data indicates that CNS tissue restorative effects are mediated by MSCs secretome, that is, the panel of bioactive factors and vesicles, with neuroregulatory properties, released by these cells to the extracellular environment [10, 19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42]
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