Abstract
The development of new technologies for introducing exogenous DNA into parasites has allowed the study of protein function, mechanisms of gene expression and its regulation, drug resistance and designing of new potential chemotherapeutic targets. The use of these modern technologies was the main objective of the Second Buriti Workshop on Transfection of DNA into Parasites, that took place at the Laboratory for Multidisciplinary Research on Chagas Disease, Faculty of Health Sciences of the University of Brasilia, from the 1st to 15 of July, 1995. The Workshop included thirteen investigators from various Brazilian and foreign research institutions, and an equal number of students that were selected from the candidate applicants from Sao Paulo, Minas Gerais, Goias and Pernambuco, and the Federal District.The theoretical aspects of the Workshop were introduced by Nancy R. Sturm (Department of Microbiology and Immunology, UCLA School of Medicine), who discussed the different methods of transfection along with requirements for electroporation and the various experimental conditions defined in the Workshop protocols. The speaker also discussed some peculiar biological features of kinetoplastid protozoa such as miniexon gene array, polyadenylation and codon usage, and polycistronic sequences and maturation of mRNA.John M. Kelly (London School of Tropical Medicine) reported on the vector he designed for transfection of
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