The SDH effects on Skeletal Muscle of Rats during Intermittent Hypoxia Training

Abstract

By the intermittent hypoxic training of 72 male SD rats, we observed the changes of SDH activity in skeletal muscle in a quiet state, immediately after exercise and three hours later. We found that in these three states , SDH in rat skeletal muscle were significantly changed, and showed a downward trend after the first rise , including hypoxia plus exercise group the most significant changes . Introduction Currently, research Hypoxic Training on Aerobic Capacity is one of the characteristics of research scholars, but hypoxic training for aerobic metabolism enzymes in rat skeletal muscle research is not common. This experiment established the intermittent hypoxic training model of SD rats for a period of four weeks of intermittent hypoxic training, observed in rat skeletal muscle succinate dehydrogenase in a quiet state, immediately after exercise, and three hours after exercise changes, provide a reference for the scientific conduct of hypoxic training. Research Content and Methods SD male rats were 72, 8 weeks old, according to the experimental animals fed standard score cages, habilitation one week after the experimental animals were randomly divided into four groups, formal training in three weeks, a total of four weeks . Experimental Methods After randomly divided into four groups of rats habilitation week : normoxic control group (C), normoxic exercise group (S), intermittent hypoxia group (I), intermittent hypoxic training group (IS) , each group 18 . Each group training arrangements 1 normoxic control group (C): normal eating, drinking freely without any training activities. 2 normoxic exercise group (S): rats only aerobic endurance training every day. 3 intermittent hypoxia group (I): hypoxic hypoxia group were in a small room in the IHT, 3 times a week, one hour a day ( a combination of hypoxia for five minutes , five minutes a batch, a total of six combinations). The concentration of oxygen in the first week was 14%, after decreasing by week, decreasing 1% per week. 4 Intermittent Hypoxic Training Group (IS): hypoxic hypoxia group were in a small room in the IHT, 3 times a week, one hour a day ( a combination of hypoxia for five minutes , five minutes a batch, a total of six combinations) . The concentration of oxygen in the first week was 14%, after decreasing by week, decreasing 1% per week of aerobic endurance training immediately after hypoxic training every day. International Conference on Information Sciences, Machinery, Materials and Energy (ICISMME 2015) © 2015. The authors Published by Atlantis Press 2113 Sport mode: exercise group ran an electric station with domestic rodents trained to adapt to the peripheral speed of 15m / min, 30min a day, the animals were familiar with treadmill exercise, one week after the start of incremental load by week, the weekly increase speed 5m / min, increase the time 10min; the last week of speeds up to 30m / min, time up to 60min. Experimental drawn. After the end of the training program, with 0.4% sodium pentobarbital lml / 100g of body weight rats were anesthetized, quickly removed the gastrocnemius and heart , the heart was weighed on Libra , and then use the left and right ventricles separated ophthalmology small scissors , measuring left with a vernier caliper ventricular wall thickness , the gastrocnemius muscle and cardiac muscle into the -70 C refrigerator for later use. Testing Index. Succinate dehydrogenase (SDH): According to Nanjing Jiancheng succinate dehydrogenase (SDH) test kit instructions for testing Test Indexes. Weigh accurately myocardium , right after the gastrocnemius each 200mg, were placed in a small beaker 5ml ; previously taken with a pipette in a refrigerator (4 C) 9% saline 1ml pre-chilled beaker , ophthalmology small scissors cut it into pieces as soon as possible organization blocks ( small beaker in ice water ) . Shredded tissue homogenates will be poured into a glass tube, then take 0.8ml cold saline flush left in the beaker tissue fragments together into homogenized tube homogenized , enabling organizations to fully homogenized , made 10 % homogenates . The homogenates prepared using a refrigerated centrifuge 3000r / min centrifugal 10min, the supernatant , succinate dehydrogenase activity was measured in accordance with succinate dehydrogenase kit . Data processing. The results are used all the data mean and standard deviation (X ± SD) to represent the statistical processing and analysis using ANOVA method, all figures are calculated on a computer using the statistical software SPSS, a significant difference was P <0.05. Experimental results. As can be seen in skeletal muscle succinate dehydrogenase changes from Table 1 : Under intermittent hypoxia modes: quiet state of intermittent hypoxia training group and normoxic control group, normoxic exercise group , intermittent hypoxia control group there was a significant difference (P <0.01), intermittent hypoxia in the control group had no significant difference; the state immediately after exercise and intermittent hypoxia group normoxic control group, normoxic exercise group showed significant differences (P <0.05), intermittent hypoxic training group and the control group normoxic, normoxic exercise group were there was a significant difference (P <0.01); the next three hours post-exercise state intermittent hypoxia group with normoxic control group there was a significant difference (P <0.01), intermittent hypoxia control group and normoxic exercise group had significantly difference (P <0.05), intermittent hypoxic training group normoxic control group, normoxic exercise group there was a significant difference (P <0.01). Table 1: Two Models of Hypoxia in rat skeletal muscle succinate dehydrogenase affected units : U / mgprot groups quiet state immidiately after exercise 3 hours later C group 2.62+0.23 2.96+0.88 2.20+0.89 S group 2.85+0.08 2.42+1.08 3.18+0.66 I group 3.17+0.45 4.69+0.94#@ 4.45+0.50##@ ISGroup 4.08+0.76##@@△△ 5.13+0.69##@@☆ 4.71+1.19##@@ Note: ( 1 ) with normoxic control group # shows P <0.05, ## shows P <0.01; (2 ) Comparison with normoxic exercise group @ shows P <0.05,@@ shows P <0.01; (3 ) and intermittent hypoxia control group shows △ P <0.05, △△ sh (4 ) and intermittent hypoxic training group * shows P <0.05, ** shows P <0.01;