Abstract
Their ease of growth and transfection makes HEK293 cells a common cell culture in academic research. In addition, high transfection efficiency of HEK293 cells enable production of exogenous proteins or viruses for pharmaceutical and biomedical research purposes. Recently, HEK293 cells has gained attention due to it is versatility for transfection experiments, particularly the propagation of adenoviral-based and retroviral-based vectors during CART-T bioprocess. Since traceability is critical to pharmaceutical manufacturing process, we provide a mini review to clarify the historical development and intent use of different variants of HEK293 cells. This review should provide a key reference for the HEK293 variants’ historical and developmental background.
Highlights
Their ease of growth and transfection makes HEK293 cells a common cell culture in academic research
Since traceability is critical to pharmaceutical manufacturing process, we provide a mini review to clarify the historical development and intent use of different variants of HEK293 cells
The microarray study demonstrate that HEK293 cells express a multitude of neuron-specific genes, indicating that 293 cells were originated from an immature neuronal cell in the embryonic kidney 10
Summary
Their ease of growth and transfection makes HEK293 cells a common cell culture in academic research. HEK293 cells has gained attention due to it is versatility for transfection experiments, the propagation of adenoviral-based and retroviral-based vectors during CART-T bioprocess. High transfection efficiency of HEK293 cells enable production of exogenous proteins or viruses for pharmaceutical and biomedical research purposes [1,2,3]. 1,4,5 Recently, HEK293 cells has gained attention due to it is versatility for transfection experiments, the propagation of adenoviral-based and retroviralbased vectors during CART-T bioprocess [6,7,8,9].
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