The S3a ribosomal protein gene is identical to the Fte-1 (v- fos transformation effector) gene and the TNF-α-induced TU-11 gene, and its transcript level is altered in transformed and tumor cells

Abstract

Previous work on mouse×rat hybrid cells (BS series) led to the assignment of a transformation suppressor locus ( Sai1) to the rat 5q22-q33 region. This gene is not yet identified. From a non-transformed BS hybrid cell line, we isolated a partial cDNA insert (13T), which detects a transcript more abundant in transformed cells than in their non-transformed homologs. Sequence comparisons led us to conclude that 13T is identical to the coding sequences of the ribosomal protein S3a gene ( Rps3a), of Fte-1 (v- fos transformation effector gene) and of TU-11, a mouse gene induced by TNF-α. Rps3a, Fte-1 and TU-11 are thus one and the same gene. Similarity was also found between this gene and non-mammalian sequences reported to be involved in cell cycling. Like the Rps3a transcript level, the c- Fos transcript level is higher in transformed cells. Rps3a and Fos could thus be effectors of the transformed phenotype.