The role of vitamin C in alteration of enzymes responsible of energy metabolism induced by administration of tamoxifen to mouse

Zaina Zuhair,Hasan Al-Amri

doi:10.4236/abc.2011.12003

Abstract

Tamoxifen is a synthetic non-steroidal ant estrogen. It was suggested to study the role of vitamin C in alte- ration of enzymes responsible of energy metabolism induced by administration of tamoxifen to mouse. The effect of tamoxifen and tamoxifen with vitamin C on some activity of enzymes in the mice representing gly- colytic, gluconeogenic and glycogenolytic pathway and also, liver function enzymes represented by aspartate aminotransferase (AST), alanine amino-transferase (ALT), acid phosphatase (ACP) and alkaline phos- phatase (ALP) were studied. The present results showed that a significant (p < 0.001) increase in glycolytic enzymes (HK, PK, GPI and PFK), glu- coneogenic enzymes, G-6-Pase, acid phosphatase (ACP), alkaline phosphatase (ALP) and glucose, were observed in treated groups, while LDH, glycogen phos- phorylase, AST and ALT enzymes activities showed significant (p < 0.01) reduction. The present results also, showed that significant reduction in glycogen, total protein, total cholesterol, uric acid, urea, and creatinine in treated mice as compared to the normal healthy control group. However, normal control mice treated with tamoxifen and vitamin C showed no side effects of most parameters compared to the normal healthy control group. It was concluded that vitamin C may prevent tamoxifen-induced testes toxicity in mice.

Highlights

Tamoxifen is one of the most effective synthetic nonsteroidal ant estrogenic compounds, it is widely used in the treatment of advanced hormone-dependent breast cancer which is the most worldwide common form of cancer in women [1] by binding to estrogen receptors and suppressing epithelial proliferation [2,3] and as adjuvant therapy following surgery in early stages of the disease
It was suggested to study the role of vitamin C in alteration of enzymes responsible of energy metabolism induced by administration of tamoxifen to mouse
The effect of tamoxifen and tamoxifen with vitamin C on some activity of enzymes in the mice representing glycolytic, gluconeogenic and glycogenolytic pathway and liver function enzymes represented by aspartate aminotransferase (AST), alanine amino-transferase (ALT), acid phosphatase (ACP) and alkaline phosphatase (ALP) were studied

Summary

Introduction

Tamoxifen is one of the most effective synthetic nonsteroidal ant estrogenic compounds, it is widely used in the treatment of advanced hormone-dependent breast cancer which is the most worldwide common form of cancer in women [1] by binding to estrogen receptors and suppressing epithelial proliferation [2,3] and as adjuvant therapy following surgery in early stages of the disease. Tamoxifen is proposed for the prevention of cancer amongst high risk women [4]. Such an approach requires objective and accurate evolution of the side effects which could result from the administration of this drug. Some studies showed that tamoxifen has adverse side effects on the cardiovascular system, bone metabolism and liver. Tamoxifen caused cytotoxicity on primary cells from human multiple organs: Kidney, liver and lung [5]. Vitamin C is an antioxidant agent that limits the injury produced by drugs. Vitamin C is an essential nutrient that functions as a non-enzymatic antioxidant in the cytosol. The various experimental studies indicated that this vitamin is effective in preventing the oxidative renal damage and stress [6]

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Conclusion