Abstract
Mixed lymphocyte cultures employing human lymphocytes were established in serum-supplemented medium. After an initial incubation of 72 hr or longer, proliferation of the cultures was observed in serum-free medium for an additional 24–48 hr. Thereafter, the proliferation decreased to below values seen in autologous controls. No DNA synthesis was observed in serum-free cultures or in cultures initially incubated with serum for less than 72 hr. Human albumin added to the cultures could not replace serum, although a weak response was obtained. Serum therefore must be present throughout the incubation period in human mixed lymphocyte cultures, if a maximum response is to be elicited.
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