Abstract
A comparative study of transfection with four different phage DNAs is being presented. Two types of transfection systems are distinguished, one with nearly linear dependence of the number of infective centers produced on the concentration of the phage DNA, the other type displaying multihit dose response. Studies of genetic recombination in transfection show that in systems of the latter type two (SPP 1) or three (SP 50) input genomes have to cooperate in a recombination event prior to replication. This obligatory process, termed primary recombination, is exclusively mediated by the host recombination system and cannot be effected by the phage recombination system.
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