Biased AC Electroosmosis Device for the Detection of DNA Molecules with Piezoresistive Microcantilever

Abstract

This work represents active DNA concentration on piezoresistive microcantilever using biased AC electroosmosis. Two different sizes of DNA molecules, -phage DNA (48.5 kbp) and single-strand DNA of 20 nucleotides are concentrated on separate piezoresistive microcantilever sen- sor for detection. Before concentration, mixing of DNA molecules is performed using the same device for efficient concentration. During concentration of DNA molecules, biased and unbiased AC elec- troosmosis are needed to concentrate single-strand DNA and -phage DNA, respectively. Moreover, dielectrophoresis is induced by biased AC electroosmosis that can generate attractive force to trans- port negatively charged single-strand DNA. This paper represents a device configuration to integrate biased and unbiased AC electroosmosis and dielectrophoresis with piezoresistive microcantilever sen- sors for the detection of -phage DNA and single-strand DNA.