Abstract
In Saccharomyces cerevisiae, the PAH1‐encoded phosphatidate phosphatase, which catalyzes the dephosphorylation of phosphatidate to yield diacylglycerol, plays a crucial role in the synthesis of the storage lipid, triacylglycerol, and in the regulation of phospholipid synthesis. The viability of yeast in stationary phase is closely related with functions of mitochondria. In this study, we showed that the pah1Δ mutant had a significantly shorter chronological life span than wild type when grown in synthetic growth medium and incubated in water. In the absence of fermentable carbon sources, the pah1Δ mutant utilized ethanol, but had a great defect in the utilization of glycerol and lactate as a non‐fermentable carbon source. Moreover, the pah1Δ mutant exhibited higher sensitivity to hydrogen peroxide which is known to generate reactive oxygen species. These results suggest that the role of PAH1‐encoded PA phosphatase is involved in cell viability that is mediated by the mitochondrial function. Supported by NIH grant GM 28140.
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