Abstract
The perilipins are a family of 5 related proteins that have been shown to play important roles in the regulation of neutral lipid metabolism. These include the interaction of lipases and acyltransferases with the lipid storage droplet and dispersion, the fragmentation of the lipid storage droplet. Recent studies have shown that perilipin 5 exists in at least two cellular pools: the lipid storage droplet and the cytosol. We hypothesized that the cytosolic pool of perilipin 5 would translocate to the lipid storage droplet when the cells were presented with a lipid challenge. Immunofluorescence microscopy was utilized to assay movement of perilipin 5. Using a transfected CHO cell model, we were able to visualize fluorescently stained lipid storage droplets within 2 hours of incubation with oleic acid. Cells were pre‐treated with cycloheximide for 30 minutes then incubated for an additional 2 hours in oleic acid to promote lipid storage. Preliminary results indicate that the cytosolic pool of perilipin 5 translocated to the storage lipid droplets. These findings show that perilipin 5 plays a role in processing neutral lipid and development of the mature lipid storage droplet.
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