Abstract
Based on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated previously that the droplet coat protein, adipose differentiation-related protein (ADFP), mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids. The present studies were designed to test the role of ADFP in this transfer with the use of ADFP-coated lipid droplets from CHO fibroblast cells and a cultured human lung epithelial cell line. We found no role for ADFP in the lipid transfer and conclude that a lipase associated with the lipid droplets hydrolyzes their core triacylglycerols, releasing fatty acids that are taken up by the epithelial cells.
Highlights
Based on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated previously that the droplet coat protein, adipose differentiation-related protein (ADFP), mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids
This project was based on an earlier study suggesting that lipid droplets from primary intracellular lipofibroblasts were transferred to type 2 lung epithelial cells via an ADFP-mediated process
Its relatively high mRNA expression in lung [3] was traced to lung lipofibroblasts [8], cells that are thought to package lipids for transfer to type 2 epithelial cells to be used for surfactant phospholipid synthesis [9]
Summary
Based on data developed with the use of isolated lipid droplets from neonatal rat lung lipofibroblasts, we speculated previously that the droplet coat protein, adipose differentiation-related protein (ADFP), mediated the transfer of lipids into type 2 lung epithelial cells for the production of surfactant phospholipids. Our group later found that high expression of ADFP occurs in lung lipofibroblasts [8] These cells are thought to store fatty acids in lipid droplets before their transfer to type 2 epithelial cells, which use the lipids to synthesize lung surfactant [9]. We discovered that isolated ADFP-coated lipid droplets were an efficient substrate for the transfer of fatty acids into type 2 cells and incorporation into surfactant phospholipids [8]. Mertz contributed to this work. 2 To whom correspondence should be addressed
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