Abstract
Cytokine-induced suppressor of cytokine signaling (SOCS) proteins function as feedback inhibitors of cytokine receptor signaling by inhibiting the Janus kinase–signal transducer and activator of transcription (JAK–STAT) signal transduction pathway. In this report, microtubule-associated protein 1S (MAP1), a member of the MAP1 family, was identified as a novel SOCS3 interacting protein. MAP1S could bind with microtubules and actin, and decorated and stabilized microtubules. A perinuclear co-localization was discovered between MAP1S and SOCS3. In MAP1S deficient macrophages, inhibition of SOCS3 on STAT3 phosphorylation can be partially hindered in the presence of interleukin-6 (IL-6) and lipopolysaccharide (LPS). The microtubule-depolymerizing drug nocodazole also disrupted the inhibitory activity of the SOCS3 protein. These results suggest that the interaction of SOCS3 with MAP1S and the integrity of the microtubule cytoskeleton play an important role in the negative regulation of SOCS3 on IL-6 signaling. Structured summary MINT- 6796998: SOCS3 (uniprotkb: O35718) physically interacts (MI: 0218) with MAP1S (uniprotkb:Q66K74) by anti bait coimmunoprecipitation (MI: 0006) MINT- 6796957: SOCS3 (uniprotkb: O14543) physically interacts (MI: 0218) with MAP1S (uniprotkb: Q66K74) by pull down (MI: 0096) MINT- 6796909, MINT- 6796875, MINT- 6796894, MINT- 6796936: SOCS3 (uniprotkb: O14543) physically interacts (MI: 0218) with MAP1S (uniprotkb: Q66K74) by two hybrid (MI: 0018) MINT- 6797017: MAP1S (uniprotkb: Q8C052) physically interacts (MI: 0218) with SOCS3 (uniprotkb: O35718) by anti bait coimmunoprecipitation (MI: 0006) MINT- 6796979: MAP1S (uniprotkb: Q66K74) and SOCS3 (uniprotkb: O14543) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416)
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
